One of the most important lessons we have learned the last years, is that the determination of the sequence is only the first step to understand genome function. Mutations are an extreme case of gene modulation; most common changes in the genome function consist in fluctuations in gene expression, temporary silencing or temporary activation. Several mechanisms modulate genome function: At a transcriptional level, the availability of the transcription factor machinery at specific sites. Epigenetic modifications are also capable to modify the genetically encode information, for example via histone modifications or DNA methylation. Finally, changes in higher-order chromatin organization and gene positioning within the nucleus alter functional properties of genome regions.
The existence of mechanisms that modulate the output of genomes makes it clear that a true understanding of genome function requires integration of what we have learned about genome sequence with what we are still discovering about how genomes are modified and importantly how they are organized in vivo in the nucleus.
What it is happening in terms of chromatin dynamics and 3D chromatin architecture in cancer cells or better, in the acquisition of malignant traits (Cancer and EMT) it is poorly understood.
Our main research objective is focused on the characterization of chromatin dynamics in cancer and in EMT. Specifically, our main aims are the following:
1. Herranz N, Dave N, Millanes-Romero A, Morey L, Díaz V, Lórenz-Fonfría V, Gutierrez-Gallego R, Jerónimo C, Di Croce L, García de Herreros A and Peiró S. LOXL2 is a Histone H3 K4 deaminase. Molecular Cell (2012). Corresponding author. Cover and a Preview. More
2. Millanes-Romero A, Herranz N, Loubat J, Iturbide A, Perrera V, Gil J, Jenuwein T, García de Herreros A and Peiró S. Regulation of Heterochromatin Transcription by Snail1/ LOXL2 During Epithelial to Mesenchymal Transition. Molecular Cell (2013). More